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Part:BBa_K1433022:Design

Designed by: Chaofan Zhang   Group: iGEM14_ZJU-China   (2014-10-16)
Revision as of 17:45, 17 October 2014 by Lilina (Talk | contribs) (→‎Source)


rT-rKan-rRBS-attB-P-attP-RBS-lambda red-RBS-Chl-T-P-RBS-gp47-tag-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1014
    Illegal NheI site found at 1037
    Illegal NheI site found at 5007
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4946
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 961
    Illegal NgoMIV site found at 5041
    Illegal AgeI site found at 2517
    Illegal AgeI site found at 4781
    Illegal AgeI site found at 5809
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 981
    Illegal BsaI.rc site found at 1064
    Illegal SapI site found at 4763


Design Notes

no


Source

Derive from 2014 Distribution Kit and pKD46 plasmid.

References