Part:BBa_K1433022:Design

rT-rKan-rRBS-attB-P-attP-RBS-lambda red-RBS-Chl-T-P-RBS-gp47-tag-T

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1014
  Illegal NheI site found at 1037
  Illegal NheI site found at 5007
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4946
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 961
  Illegal NgoMIV site found at 5041
  Illegal AgeI site found at 2517
  Illegal AgeI site found at 4781
  Illegal AgeI site found at 5809
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 981
  Illegal BsaI.rc site found at 1064
  Illegal SapI site found at 4763
  

Design Notes

no

Source

Derive from 2014 Distribution Kit and pKD46 plasmid.

References

1. Baba T, Ara T, Hasegawa M, et al. Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection[J]. Molecular systems biology, 2006, 2(1).
2. Mosberg J A, Lajoie M J, Church G M. Lambda red recombineering in Escherichia coli occurs through a fully single-stranded intermediate[J]. Genetics, 2010, 186(3): 791-799.
3. Datsenko K A, Wanner B L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products[J]. Proceedings of the National Academy of Sciences, 2000, 97(12): 6640-6645.
li>Bonnet J, Subsoontorn P, Endy D. Rewritable digital data storage in live cells via engineered control of recombination directionality[J]. Proceedings of the National Academy of Sciences, 2012, 109(23): 8884-8889.
4. Siuti P, Yazbek J, Lu T K. Synthetic circuits integrating logic and memory in living cells[J]. Nature biotechnology, 2013, 31(5): 448-452.
5. Sharan S K, Thomason L C, Kuznetsov S G, et al. Recombineering: a homologous recombination-based method of genetic engineering[J]. Nature protocols, 2009, 4(2): 206-223.