Regulatory
AraC

Part:BBa_R0080

Designed by: Sara Neves (Fighting Darwins)   Group: Antiquity   (2004-01-27)
Revision as of 03:35, 17 October 2014 by Qiuxinyuan12 (Talk | contribs) (→‎Contribution: iGEM14_NUDT_CHINA)

Promoter (AraC regulated)

AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC or the simple sugar [http://openwetware.org/wiki/Arabinose arabinose]

b/c the operator lacks the araO2 site, there should not be araC-mediated repression.


Usage and Biology

araC binding region from E.coli


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Contribution: iGEM14_NUDT_CHINA

Group: iGEM14_NUDT_CHINA/ Author: Xinyuan Qiu

Summary: We tested expression of this part in E.coli, and corrected the founction description of this part.

Methods and Practices

[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
biology
controlaraC
directionForward
negative_regulators
o_h
o_l
positive_regulators1