Coding

Part:BBa_K1442308:Design

Designed by: Caroline de Cock   Group: iGEM14_Warwick   (2014-10-15)
Revision as of 22:48, 15 October 2014 by CarriedC (Talk | contribs) (→‎Design Notes)


GFP codon optimised for human cells


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 44


Design Notes

Codon optimised using IDT codon optimiser

This contains restriction sites in order to insert testing modules either side of the forwards GFP.

The stop codons were artificially added in order to definitively stop translation following this.

Source

Part BBa_E0040

References