Device

Part:BBa_K1554005:Design

Designed by: Valencia_UPV team members   Group: iGEM14_Valencia_UPV   (2014-09-29)
Revision as of 19:07, 15 October 2014 by Lestelles (Talk | contribs)

Blue biosafety module for plants


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3097
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The assembly was performed by GoldenBraid 2.0, a Golden-Gate based assembly system. The barnase was amplified by PCR from Part:BBa_J72173 and a mutation in T105P was introduced.

Source

TA29 promoter Part:BBa_K1554000

Barnase Part:BBa_J72173

AmilCP Part:BBa_K1467202

P35s Part:BBa_K1537015

T35s: GoldenBraid 2.0 Collection

References