Coding

Part:BBa_K1499400:Design

Designed by: Ian Hull   Group: iGEM14_StanfordBrownSpelman   (2014-10-09)
Revision as of 17:23, 10 October 2014 by Ianhull (Talk | contribs) (Source)

Chitin-binding wasp protein 1 (PdomMRNAr1.2-03231.1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 143
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for this gene was predicted through peptide mass fingerprinting of Polistes dominula nest protein samples. We believe it to be a potential candidate for the salivary protein that the wasps use to build and waterproof their nests. We have codon-optimized it for E. coli expression.


Source

Polistes dominula genome.

References