Translational_Unit

Part:BBa_K1465228:Design

Designed by: iGEM-Team Bielefeld 2014   Group: iGEM14_Bielefeld-CeBiTec   (2014-10-05)
Revision as of 23:14, 8 October 2014 by Tore (Talk | contribs) (→‎Design Notes)


Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw-pSB1C3-BBa_B0034-SBPase fw-pSB1C3-BBa_B0034-SBPase] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv-SBPase-BBa_B0034-pSB1C3 rv-SBPase-BBa_B0034-pSB1C3]:
fw-pSB1C3-BBa_B0034-SBPase (58 bp)

5'-GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAGGGAATTGAAAAGCGAAAAG-3'

rv-SBPase-BBa_B0034-pSB1C3 (65 bp)

5'-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3'
  • Amplification of the front part of the Sedoheptulose-1,7-Bisphosphatase (glpX) for the removal of the illegal recostriction site. This part contains the RBS BBa_B0034 and the front part of the Sedoheptulose-1,7-Bisphosphatase from Bacillus metanolicus. For the amplification the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_pSB1C3_csoS4A csoS4A_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_csoS4A_PstI csoS4A_PstI-rev] were used:

Source

Gibson

References