Protein_Domain
SspDnaE(N)

Part:BBa_K1362410:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-06)
Revision as of 18:49, 6 October 2014 by Jakob (Talk | contribs) (→‎References)

SspDnaE N-Intein cloning piece


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.


Source

Part sequence and DNA were obtained from Addgene (pSFBAD09) [1]. Further Information came amongst other literature from Iwai et al. [2]

References

[1] https://www.addgene.org/11962/

[2] Sara Züger & Hideo Iwai Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies Nature Biotechnology 23, 736 - 740 (2005) Published online: 22 May 2005 | doi:10.1038/nbt1097"