Protein_Domain
SspDnaE(N)
Part:BBa_K1362410:Design
Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha Group: iGEM14_Heidelberg (2014-10-06)
SspDnaE N-Intein cloning piece
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part represents only the intein sequence without including the standard splicing-site or polyglycine-linker overhangs.
Source
Part sequence and DNA were obtained from Addgene (pSFBAD09) [1]. Further Information came amongst other literature from Iwai et al. [2]
References
[1] https://www.addgene.org/11962/
[2] Sara Züger & Hideo Iwai Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies Nature Biotechnology 23, 736 - 740 (2005) Published online: 22 May 2005 | doi:10.1038/nbt1097