Regulatory

Part:BBa_K1372001

Designed by: Sylvie Lautru Fabio Maschi   Group: iGEM14_Paris_Saclay   (2014-10-02)
Revision as of 13:45, 2 October 2014 by Registry (Talk | contribs)

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Salicylate Inducible Suppressing System

This briobrick is the BBa_K1372000 biobrick with a terminator added at the end. It allows the control of the expression of the SupD-tRNA (BBa_K228001) by salicylate. In presence of salicylate, the salicylate-NahR complex activates the transcription of the SupD-tRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1312


[edit]
Categories
Parameters
None