Reporter

Part:BBa_M45117

Designed by: Federico C. Rodriguez   Group: UtahState_BE5930_-_S14   (2014-04-19)
Revision as of 23:14, 19 April 2014 by Registry (Talk | contribs)

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Uranium Inducible GFPuv Reporter

This composite part is made out of the uranium inducible (urcA)promoter (BBa_M45114), a ribosome binding site, the GFPuv protein which is a green fluorescent protein (GFP) variant optimized for excitation by UV light, and an extra stop codon to prevent RNA polymerase from reading it through.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1520
    Illegal XhoI site found at 1421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2
    Illegal NgoMIV site found at 317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 630
    Illegal SapI.rc site found at 584


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