Generator

Part:BBa_K1021033

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-15)
Revision as of 00:28, 29 October 2013 by R.Ashley (Talk | contribs)

PT7+hph

The hygromycin phosphotransferase gene (hph), which also confers resistance to hygromycin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 391
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None