Generator

Part:BBa_K1021033

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-15)

PT7+hph

The hygromycin phosphotransferase gene (hph), which also confers resistance to hygromycin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi.

Usage and Biology

  • The part was constructed from BBa_I712074 and BBa_K1021002.
  • Cornell iGEM characterized this part in E. coli BL21-A1 using a zone of inhibition test. The data is displayed below; at concentrations of 200 and 2000 μg/mL, the wild-type strain was significantly more inhibited than the allegedly hygromycin-resistant strain (one-tailed student's t-test, α=0.005).

Hph.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 391
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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