Composite

Part:BBa_K1139026:Design

Designed by: Shinya Suzuki   Group: iGEM13_Tokyo_Tech   (2013-10-21)
Revision as of 16:37, 28 October 2013 by Keso57 (Talk | contribs) (→‎Source)


Pbad-M13-Plac-GFP on pSB3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 238
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7425


Design Notes

sequence confirmed

Source

BAD promoter from (BBa_I13453), M13 genes and origin from BBa_K1139018, GFP from BBa_E0040, rbs from BBa_B0030, Double terminator from BBa_B0015 are combined.

References