Part:BBa_K1031917

J23117-XylS-Terminator

For detailed information concerning XylS, please visit 2013 Peking iGEM Biosensor XylS

Characterization

In order to fine-tune the performance of XylS biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031917 is composed of three elements, the constitutive Pc promoter J23117, coding sequence of XylS and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Pm/J23117-XylS. Orange arrowheads represent promoters. RBSs(Ribosome Binding Sites) are shown as green ovals and squares in dark red stand for B0015 terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 94
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 816
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 279

Data shown

Dose response curve of biosensor circuit Pm/J23117-XylS when exposed to 4 typical inducers at different concentration. This biosensor performs well.(Fig 2)

Fig 2 4 inducers are shown in different colors. Horizontal axis stands for different concentration of BzO, 2-,3-,4-BzO. The test was following Test Protocol 1. Vertical axis represents the induction ratio calculated from fluorescence intensity.