Composite

Part:BBa_K737024:Experience

Designed by: Minghao Gong   Group: iGEM12_OUC-China   (2012-09-18)
Revision as of 16:05, 28 October 2013 by Sarasate (Talk | contribs) (→‎User Reviews)


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Applications of BBa_K737024

User Reviews

UNIQ5f6c357bb0d21acd-partinfo-00000000-QINU UNIQ5f6c357bb0d21acd-partinfo-00000001-QINU

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Tokyo-Tech iGEM 2013

1. Sequence
No NotI restriction site in prefix and suffix.
1bp deletion and 2bp mutation exist between the promoter and RBS.

2. Result of our experiment
We improved this OUC-China’s phosphate sensor part including the phoB promoter, by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part (BBa_K1139200)

Fig. 1. Our phoA promoter assay result
Fig. 2. OUC-China 2012’s phoB promoter assay result (converted to bar chart)













Compared to OUC-China’s phosphate sensor part including phoB promoter (Fig. 2), our phosphate sensor part shows clearer result (Fig. 1) (BBa_K1139200, BBa_K1139201).