Composite

Part:BBa_K737024:Experience

Designed by: Minghao Gong   Group: iGEM12_OUC-China   (2012-09-18)

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Applications of BBa_K737024

User Reviews

UNIQd3e49eba624e194c-partinfo-00000000-QINU UNIQd3e49eba624e194c-partinfo-00000001-QINU

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Tokyo-Tech iGEM 2013

1. Sequence
No NotI restriction site in prefix and suffix.
1bp deletion and 2bp mutation exist between the promoter and RBS.

2. Result of our experiment
We improved this OUC-China’s phosphate sensor part including the phoB promoter, by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part (BBa_K1139201)

Fig. 1. Our induction assay result for our phoA promoter
Fig. 2. Our induction assay result for OUC-China 2012's phoB promoter













Compared to OUC-China’s phosphate sensor part including the phoB promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (BBa_K1139200, BBa_K1139201).