Device
Part:BBa_K1104250
Designed by: Ting-Yun Chiang Group: iGEM13_NYMU-Taipei (2013-10-27)
OxyR device
In order to enhance the strength and sensitivity of ROS-sensing promoters, we make E. coli continuously produce inactivated OxyR, so that ROS can affect promoters more easily by adding a device: Device1.
Device1 in order to enhance the effect of ROS on E. coli is composed of :
- a constitutive promoter(Part:BBa_J23102)
- RBS(Part:BBa_B0034)
- activator OxyR(Part:BBa_K1104200)
- double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015
Putting Device1 device:BBa_K1104250 upstream to promoter AhpCp2D1(Part:BBa_K1104204) remarkably enhanced the strength of promoter AhpCp2D1(Part:BBa_K1104204) as our experiment results shows.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |