Composite

Part:BBa_K1232000

Designed by: Austin Moon, Elsie Sawai   Group: iGEM13_Washington   (2013-09-17)
Revision as of 20:31, 5 October 2013 by Bullzi (Talk | contribs)

Green light inducible sfGFP = [PCpcG2-RBS-sfGFP-CcaS](rev)-CcaR

This is the green light responsive GFP output system from the Jeff Tabor plasmid pJT119b.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1023
    Illegal EcoRI site found at 3898
    Illegal XbaI site found at 4599
    Illegal SpeI site found at 928
    Illegal SpeI site found at 4594
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1023
    Illegal EcoRI site found at 3898
    Illegal NheI site found at 1652
    Illegal SpeI site found at 928
    Illegal SpeI site found at 4594
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1023
    Illegal EcoRI site found at 3898
    Illegal BamHI site found at 4295
    Illegal XhoI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1023
    Illegal EcoRI site found at 3898
    Illegal XbaI site found at 4599
    Illegal SpeI site found at 928
    Illegal SpeI site found at 4594
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1023
    Illegal EcoRI site found at 3898
    Illegal XbaI site found at 4599
    Illegal SpeI site found at 928
    Illegal SpeI site found at 4594
    Illegal NgoMIV site found at 2444
    Illegal AgeI site found at 988
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3619


[edit]
Categories
Parameters
n/aGreen light inducible sfGFP = [PCpcG2-RBS-sfGFP-CcaS](rev)-CcaR