Part:BBa_K1232000

Green light inducible sfGFP = [PCpcG2-RBS-sfGFP-CcaS](rev)-CcaR

This is the green light responsive GFP output system from the Jeff Tabor plasmid pJT119b.

Usage and Biology

For the biology of this part refer to the [http://2013.igem.org/Team:Washington/LightSensing#Systems Washington 2013 wiki page.]

Native versus Biobrick systems over time.

The first pair of lines in green represent the fluorescence time course of a dual transformation of the native plasmids supplied by the Tabor Lab at Rice University in accordance to the light testing protocol described by the University of Washington iGEM 2013 team on their wiki. There is a measurable difference in GFP expression between the green light and dark conditions.

The second pair of lines in red represent the same experiment but using this biobrick (BBa_K1232000) to express the light sensing proteins and the native plasmid for expression of chromophore expression. There remains a measurable difference in GFP expression between green light and dark conditions.

The third pair of lines in blue represent, again, the same experimental setup but using BBa_K1232004 to express the chromophore producing proteins and the native plasmid for expressing light sensing proteins. Again, there is a measurable difference in GFP expression between green light and dark conditions.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1023
  Illegal EcoRI site found at 3898
  Illegal XbaI site found at 4599
  Illegal SpeI site found at 928
  Illegal SpeI site found at 4594
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1023
  Illegal EcoRI site found at 3898
  Illegal NheI site found at 1652
  Illegal SpeI site found at 928
  Illegal SpeI site found at 4594
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1023
  Illegal EcoRI site found at 3898
  Illegal BamHI site found at 4295
  Illegal XhoI site found at 6
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1023
  Illegal EcoRI site found at 3898
  Illegal XbaI site found at 4599
  Illegal SpeI site found at 928
  Illegal SpeI site found at 4594
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1023
  Illegal EcoRI site found at 3898
  Illegal XbaI site found at 4599
  Illegal SpeI site found at 928
  Illegal SpeI site found at 4594
  Illegal NgoMIV site found at 2444
  Illegal AgeI site found at 988
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 3619