Not Released
Sample In stock
Experience: Issues
Not Used
Get This Part
Regulatory

Part:BBa_K1155006:Experience

Designed by: Solenne Ithurbide, Abdou, Anas, Zhu, Xavier, Nadia   Group: iGEM13_Paris_Saclay   (2013-09-13)
Revision as of 22:08, 4 October 2013 by SolenneParisSaclay (Talk | contribs) (→‎Applications of BBa_K1155006)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1155006

We have tested the activity of BBa_K1155006 by observing the expression of lacZ.

Construction: BBa_K1155006 + BBa_K1155007 in pSB1K3 ( construction obtained not yet sequenced)

Strains were plated on LB + Xgal and incubated in anaerobic conditions.

What we expected :

+ O2 : FNR inactivated : PnarK repressed --> no color

- O2 : FNR activated : PnarK activated --> blue color

What we observed :

+ O2 : --> not tested

- O2 : --> blue color

[photo]

These results indicate that the PnarK(BBa_K1155004) seems to be well induced by FNR in anaerobic condition. The repression of these construction has not been tested in aerobic conditions.

User Reviews

UNIQ34e7c99cd44cf6b7-partinfo-00000000-QINU UNIQ34e7c99cd44cf6b7-partinfo-00000001-QINU