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Part:BBa_K1155006:Experience

Designed by: Solenne Ithurbide, Abdou, Anas, Zhu, Xavier, Nadia   Group: iGEM13_Paris_Saclay   (2013-09-13)


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Applications of BBa_K1155006

We have tested the activity of BBa_K1155006 by observing the expression of lacZ.

Construction: BBa_K1155006 + BBa_K1155007 in pSB1K3 ( construction obtained not yet sequenced)

Strains were plated on LB + Xgal and incubated in anaerobic conditions.

What we expected :

+ O2 : FNR inactivated : PnarK repressed --> no color

- O2 : FNR activated : PnarK activated --> blue color

What we observed :

+ O2 : --> not tested

- O2 : --> blue color ( both plates )

Ps007.jpg

These results indicate that the PnarK(BBa_K1155004) seems to be well induced by FNR in anaerobic condition. The repression of these construction has not been tested in aerobic conditions.

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