Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Composite

Part:BBa_K1045013:Design

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)
Revision as of 17:38, 4 October 2013 by Kati (Talk | contribs) (→‎Source)

Promoter - DarR operator - GFP generator BBa_E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 728


Design Notes

For cloning of BBa_K1045013, first, oligos for BBa_K1045012 were hybridized. The hybridization oligos were designed such that their double-stranded hybridization product would correspond to the sequence of BBa_K1045012 cut with EcoRI and SpeI at the prefix and suffix sites. Second, this hybridization product was ligated in front of the GFP generator BBa_E0240 previously cut with EcoRI and XbaI.

Source

The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of BBa_J23110 and the sequence of the DarR operator found upstream of DarR (Zhang et al., 2013). The plasmid of part BBa_E0240 derived from the distribution kit 2013.

References