Regulatory

Part:BBa_K1067007

Designed by: Kristian Davidsen and Helen Cook   Group: iGEM13_DTU-Denmark   (2013-09-28)
Revision as of 16:07, 4 October 2013 by KrDa (Talk | contribs)

Tight pBAD with araC

Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as part:BBa_K808000 but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the pBAD SPL col. 10 from [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team].

For characterization see the pBAD SPL page or go to the 2013 DTU [http://2013.igem.org/Team:DTU-Denmark/pBAD_SPL wiki].


Usage and Biology

  • Expression of lethal proteins
  • Tight control
  • Direct proteins to the periplasm, see Part:BBa_K1067009

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1182
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1182
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//cds/transcriptionalregulator/activator
//regulation/positive
Parameters
positive_regulators