Coding

Part:BBa_K1189000:Experience

Designed by: Julie Osgood   Group: iGEM13_Calgary   (2013-09-17)
Revision as of 17:45, 1 October 2013 by Lisa.O (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1189000

This protein has been modified in order to improve its expression and widen its purpose.

1. We have amended the mutation in 2012 Slovenia's TALEs such that the TALEs bind to the correct target sequence specified by that team.

2. We have optimized the expression of TALEs in E. coli. i. Codon optimized the sequence such that these TALEs can be expressed efficiently in E. coli. ii. Removed the eukaryotic kozak sequence such that it can be used in E. coli because the presence of a kozak sequence quenches expression of this protein. iii. Removed the nuclear localization sequences from the TALEs. iv. Put a KasI restriction cut site in the end of the TALE sequence to implement a plug and play system for user-customized TALEs in the future. We designed our generator such that any TALEs can be put in with just one cloning step in order to allow the easy modification of the binding target of the protein in our circuits.

User Reviews

UNIQb81824875cb688d7-partinfo-00000000-QINU UNIQb81824875cb688d7-partinfo-00000001-QINU