Part:BBa_K1189000:Experience

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Applications of BBa_K1189000

This protein has been modified in order to improve its expression and widen its purpose.

1. We have amended the mutation in 2012 Slovenia's TALEs such that the TALEs bind to the correct target sequence specified by that team.

2. We have optimized the expression of TALEs in E. coli. i. Codon optimized the sequence such that these TALEs can be expressed efficiently in E. coli. ii. Removed the eukaryotic kozak sequence such that it can be used in E. coli because the presence of a kozak sequence quenches expression of this protein. iii. Removed the nuclear localization sequences from the TALEs. iv. Put a KasI restriction cut site in the end of the TALE sequence to implement a plug and play system for user-customized TALEs in the future. We designed our generator such that any TALEs can be put in with just one cloning step in order to allow the easy modification of the promoter and binding target of the protein in this and any more complex circuits constructed with this part.

User Reviews

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