Part:BBa_K1073022
Constitutively expressed chromoprotein eforRed
The device consists of a strong promoter (BBa_J23100) combined with a RBS (BBa_B0032) and the encoding region of the chromoprotein eforRed. This results in a constitutively high expression of eforRed.
Usage and Biology
During cultivation, the chromoprotein eforRed is expressed and stored intracellularly. The pink color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter to distinguish cells easily.
iGEM Team Braunschweig 2013:
Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP (BBa_K1073024), eforRed and aeBlue (BBa_K1073020).
Right: Cell pellet of E. coli XL1 expressing eforRed.
The pink color of eforRed cannot only be seen by naked eye but it also fluoresce when excited. Thus it can be used to identify cells with fluorescent microscopy or FACS. For further information as well as absorption and emission spectra see experience page.
iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed and amilGFP (BBa_K1073024). Both chromoproteins fluoresce when excited by UV-light.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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