Composite

Part:BBa_K1127016

Designed by: Jonas Kondratavicius   Group: iGEM13_York_UK   (2013-09-15)
Revision as of 20:49, 29 September 2013 by Kobachii.d (Talk | contribs)

Gold and QS ultra complex

The device is a tunable hybrid bistable switch. It receives two signals 1. gold (HAuCl4) and 2. population density, which modify magnitude of the output 'transactivation of the target gene'.

LuxR [1] is produced constitutively by the promoter BBa_J23100 [2]. It is activated by the quorum sensing signal AHL and forms a positive feedback loop to promote expression of the AHL synthase LuxI [3] and transactivating RNA taR12 [4]. Gold is recognized by the part BBa_K1127008 [5] which in response produces AiiA [6]. AiiA can degrade AHL; therefore, it reduces expression of the target gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1747
    Illegal NheI site found at 1770
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 701
    Illegal AgeI site found at 880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2808


[edit]
Categories
Parameters
None