Part:BBa_K1127016
Gold and QS ultra complex
The device is a tunable hybrid bistable switch. It receives two signals 1. gold (HAuCl4) and 2. population density, which modify magnitude of the output 'transactivation of the target gene'.
LuxR [1] is produced constitutively by the promoter BBa_J23100 [2]. It is activated by the quorum sensing signal AHL and forms a feedback loop to promote expression of the AHL synthase LuxI [3] and the transactivator taR12 [4]. The latter binds to crR12 [5] preceding translation start site of the mRNA of the target gene resulting in full expression. Gold is recognized by the part BBa_K1127008 [6] which in response produces AiiA [7]. AiiA can degrade AHL; therefore, it reduces expression of the target gene. The mechanism is simplified and shown in Figure 1.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1747
Illegal NheI site found at 1770 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3565
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 701
Illegal AgeI site found at 880 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2808
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