Intermediate

Part:BBa_K1150063

Designed by: M. Agne, N. Gillich, A. Rudorf   Group: iGEM13_Freiburg   (2013-09-17)
Revision as of 00:00, 29 September 2013 by Ilona (Talk | contribs)

HA-NLS-Cas9-Linker 3 AA

.

HA-NLS-Cas9-Linker
Function A new effector can be fused to

this Cas9 intermediate part

Use in Mammalians
RFC standard Front RFC 10 & back RFC 25
Backbone pSB1C3
Organism Arabidopsis thaliana
Source AG Weber, Freiburg
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

Biology and Usage

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Literature

[edit]
Categories
Parameters
None