Composite
Part:BBa_K1065306:Design
Designed by: Caterina Marchioretti Group: iGEM13_UNITN-Trento (2013-09-15)
J23100+YF1+FixJ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218
Design Notes
This part was built starting from the extraction of BBa_K592016 from the iGEM Registry 2013 Distribution Kit. The part then was subcloned in No part name specified with partinfo tag. following standard assembly.
Source
This part has been built using:
BBa_J23100 a constitutive promoter from the Anderson family.
BBa_K592016 the part with RBS-coupled YF1 and FixJ.
References
1) Andreas Möglich, Rebecca A. Ayers, Keith Moffat (2009). Design and Signaling Mechanism of Light-Regulated Histidine Kinases. Journal of Molecular Biology, 1433–1444.
2) Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542