Generator

Part:BBa_K1073016

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 11:19, 28 September 2013 by TUNKI (Talk | contribs) (→‎Usage and Biology)

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Constitutively expressed transcription regulator LasR + LVA

The device consists of a strong constitutive promoter (BBa_J23100) combined with RBS (BBa_B0032) and the encoding region of the transcription regulator LasR (BBa_C0079).This results in a constitutively high transcription level of LasR.


Usage and Biology

In combination with N-3-oxo-dodecanoyl-HSL synthesized by LasI, LasR is able to activate the inducible promoter of the las quorum sensing system of Pseudomonas aeruginosa.

LasR binds to specific sequences in the promoter region. In the presence of N-3-oxo-dodecanoyl-HSL it activates gene expression (Medina et al., 2003).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 384
    Illegal AgeI site found at 581
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None