Generator

Part:BBa_K1073011:Experience

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)
Revision as of 11:12, 28 September 2013 by TUNKI (Talk | contribs) (→‎User Reviews)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073011

iGEM Team Braunschweig 2013 used this device for the contruction of BBa_K1073035. It was used in order to induce growth in the presence of RhlR and N-buturyl-HSL in ampicillin containing media.

User Reviews

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iGEM Team Braunschweig 2013

This device was successfully employed in BBa_K1073035 to mediate ampicillin resistance in the presence of RhlR and N-buturyl-HSL. Due to a low level leakiness of the promoter and the bacteriostatic properties of ampicillin, the cells will start to grow in batch cultivations when a low level of ampicillin is reached even whithout N-buturyl-HSL.Uninduced activity of the promoter differs depending on strain and media used. UNIQ9e70ca60c8c8c468-partinfo-00000004-QINU