Composite

Part:BBa_K1036005:Design

Designed by: Zhaopeng Cheng, Xiyu Wu   Group: iGEM13_XMU-China   (2013-09-09)
Revision as of 21:55, 27 September 2013 by Tina Zhang (Talk | contribs) (→‎Design Notes)


sfgfp (with LVA-tag) under plux R control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914
    Illegal BsaI.rc site found at 2017
    Illegal SapI.rc site found at 31


Design Notes

This part contains two subparts: BBa_K1036004 and BBa_K546000 (located in 2013-Plate 1-12D). BBa_K1036004 digested by EcoR I & SpeI and BBa_K546000 digested by EcoR I & XbaI were ligated together by T4 ligase.

Source

The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.

References

1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)
3. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)