Composite
Part:BBa_K1036005:Design
Designed by: Zhaopeng Cheng, Xiyu Wu Group: iGEM13_XMU-China (2013-09-09)
sfgfp (with LVA-tag) under plux R control
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2737
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 914
Illegal BsaI.rc site found at 2017
Illegal SapI.rc site found at 31
Design Notes
This part contains two subparts: RBS-sfgfp(-LVA)-TT(BBa_K1036004)and BBa_K546000 (located in 2013-Plate 1-12D). RBS-sfgfp(-LVA)-TT digested by EcoR I & SpeI and BBa_K546000 digested by EcoR I & XbaI were ligated together by T4 ligase.
Source
The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.
References
1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)
3. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)