Generator

Part:BBa_K1073011

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)
Revision as of 14:48, 26 September 2013 by Laura S (Talk | contribs)

Inducible ampicillin resistance cassette (regulated by RhlR + N-buturyl-HSL)

This device is a combination of BBa_K1073001 and No part name specified with partinfo tag. and mediates an inducible ampicillin resistance. It includes the inducible promoter of the rhl quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.

Usage and Biology

The promoter is activated in the presence of RhlR in combination with the autoinducer molecule N-buturyl-homoserine lactone produced by RhlI. The expression of the beta-lactamase can therefore be induced and tuned by supplying RhlR and N-buturyl-HSL.

Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073035.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None