Coding
lacI

Part:BBa_C0012:Design

Designed by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton   Group: Antiquity   (2003-01-31)
Revision as of 23:46, 19 July 2006 by Smelissali (Talk | contribs) (→‎References: added references to protein databases)

lacI repressor from E. coli (+LVA)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation.

Incompatible with systems containing LacI, lactose, or IPTG.

Source

represillator of Elowitz and Leibler (2000)

References

  • UniProt accession #: [http://www.pir.uniprot.org/cgi-bin/upEntry?id=LACI_ECOLI P03023; O09196; P71309; Q2MC79; Q47338]
  • Entrez Gene accession #: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=945007 945007]
  • NCBI GI accession #:[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Protein&dopt=GenPept&list_uids=g1633173 g1633173; g49176012; g1786540; g16975390; g1699071; g494230; g16975388; g146576; g1633171; g88683482; g82408115; g1127216; g1633178; g7245444; g494233; g89107217; g6730170; g1657541; g7245446; g1840130; g1633176; g1633174; g16975201; g930016; g1684865; g1127218; g1633172; g33112645; g1127217; g7245445; g1633179; g16975202; g21730604; g85674487; g1633170; g82408114; g1127215; g1633177; g1633168; g16975389; g1699067; g6730171; g1942288; g1633175; g1699063; g1633169; g581123; g21730605]
  • Protein DataBase entry: [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1CJG here]
  • Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [http://biobricks.ai.mit.edu/BB_References.htm#ELOW00 link]