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Part:BBa_K1073022:Experience

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 20:15, 24 September 2013 by Kerstin K (Talk | contribs) (→‎User Reviews)


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Applications of BBa_K1073022

When eforRed is expressed, the colonies on plates as well as the liquid culture become red. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains from one approach.

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BBa_K1073022 iGEM Team Braunschweig 2013

The chromoprotein eforRed has a distinct red color. In order to avoid absoption of the chomoproteins during measurement with the photometer, the absorption and emission spectra were measured.

BSAbsorption-eforRed.jpg

Braunschweig 2013: Absorptionspectrum of chromoprotein eforRed in the wavelength range 400-800 nm.


absorption maximum for eforRed: 534 nm

emission maximum for eforRed: 605,4 nm


Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and aeBlue (BBa_K1073020).

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