Composite
Part:BBa_K1045012
Designed by: iGEM Team Göttingen 2013 Group: iGEM13_Goettingen (2013-09-20)
Promoter with DarR operator
This composite part is composed of a strong promoter (BBa_J23110) derived from the Anderson collection and a downstream sequence of the DarR operator (BBa_K1045000). It could be placed upstream of a protein coding sequence with an RBS in front of it. If the DarR repressor (for more information, see BBa_K1045001) is available, as well, the expression of this protein coding sequence might be regulated by DarR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
n/a | Promoter with DarR operator |