Part:BBa_K1031912

J23105-XylS-Terminator

Characterization

In order to fine-tune the performance of XylS biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031912 is composed of three elements, the constitutive Pc promoter J23105[1], coding sequence of XylS and terminator B0015[2].

Fig1 Construction of biosensor circuit ==='''Usage and Biology'''=== Sequence and Features BBa_K1031912 SequenceAndFeatures ==='''data'''=== '''Fig 2''' Induction ratio of XylS biosensor library from ON/OFF test that utilizes different Pc promoters to control the expression of XylS. Horizontal axis stands for different XylS biosensor with Pc promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Partsregistry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following Test Protocol 1 (a hyper link is needed here). Vertical axis represents the ON-OFF induction ratio. The Pm/XylS expression system which adopted Pc promoter J23114 clearly performed best of all of the four inducers.