Generator

Part:BBa_K1076002:Design

Designed by: Carlos Gustavo Nunes   Group: iGEM13_Manaus_Amazonas-Brazil   (2013-09-13)
Revision as of 23:38, 19 September 2013 by Lais (Talk | contribs) (→‎Design Notes)


FadL, Long-chain fatty acid outer membrane transporte


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 157
    Illegal PstI site found at 288
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 157
    Illegal PstI site found at 288
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1021
    Illegal BamHI site found at 1765
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 157
    Illegal PstI site found at 288
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 157
    Illegal PstI site found at 288
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chosed the full lenght gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3. Further subclonning and expression should be done within pSB3C5-152001.

Source

From Shewanella putrefaciens genomic sequence

References