Part:BBa_K1076002:Design
FadL, Long-chain fatty acid outer membrane transporte
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 157
Illegal PstI site found at 288 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 157
Illegal PstI site found at 288 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1021
Illegal BamHI site found at 1765 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 157
Illegal PstI site found at 288 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 157
Illegal PstI site found at 288 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We chosed the full lenght gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3. Further subclonning and expression should be done within pSB3C5.
Source
From Shewanella putrefaciens genomic sequence
References
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