Composite

Part:BBa_K1030002

Designed by: Wei Huang   Group: iGEM13_SUSTC-Shenzhen-A   (2013-09-16)
Revision as of 02:55, 17 September 2013 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


J23102->tetA-asv+sfGFP

We use a synthetic constitutive promoter J23102 to drive the expression of tetA, and sfGFP. tetA could transport extracellular Nickel ion into cell and nickel is toxic to E coli. An asv degradation tag is added to the c-terminal of tetA. sfGFP is used to monitor tetA expression in real time using fluorescent plate reader, flow cytometer and microscope.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 406
    Illegal NgoMIV site found at 566
    Illegal NgoMIV site found at 934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1273


[edit]
Categories
Parameters
None