Coding
MarA

Part:BBa_K1230000:Design

Designed by: Andrés Ferriño Iriarte   Group: iGEM13_Ciencias-UNAM   (2013-09-08)
Revision as of 05:26, 8 September 2013 by Andresferrino (Talk | contribs) (→‎Design Notes)


AcrAB-TolC multidrug efflux transport system activator from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 154
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutation from G to C at base 132 to remove AarI and BspMI sites.

Source

Synthesis from genscript

References