Part:BBa_K1230000:Design

AcrAB-TolC multidrug efflux transport system activator from E. coli

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 154
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Silent mutation from G to C at base 132 to remove AarI and BspMI sites.

Source

Synthesis from genscript

References

1. Maneewannakul, K. & Levy, S. B. (1996). Identification for mar mutants among quinolone-resistant clinical isolates of Escherichia coli. Antimicrob Agents Chemother 40, 1695–1698. 2. Warner, D.M.& Levy, S.B., "Different effects of transcriptional regulators MarA, SoxS and Rob on susceptibility of Escherichia coli to cationic antimicrobial peptides (CAMPs): Rob-dependent CAMP induction of the marRAB operon", Microbiology, vol. 156, 2010, p.570-578. 3. Nikaido, H. (2003). Bile salts and fatty acids induce the expression of Escherichia coli AcrAB multidrug efflux pump through their interaction with Rob regulatory protein. Mol Microbiol 48, 1609–1619. 4. Wandersman, C. & Delepelaire, P. (1990). TolC, an Escherichia coli outer membrane protein required for hemolysin secretion. Proc Natl Acad Sci U S A 87, 4776–4780.