Translational_Unit
AtIPT4

Part:BBa_K1139100:Design

Designed by: Naoki Watarai   Group: iGEM13_Tokyo_Tech   (2013-08-29)
Revision as of 14:14, 1 September 2013 by Tryalnigro (Talk | contribs) (→‎Source)


Adenylate isopentenyltransferase 4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cds is exactly the same as IPT4 gene sequence of Arabidopsis thaliana. The RBS is [1] (efficiency 0.6).

Source

http://www.ncbi.nlm.nih.gov/gene/828567

References