Device

Part:BBa_K1065102

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-08-28)
Revision as of 15:45, 31 August 2013 by TULIO007 (Talk | contribs)

Wintergreen device: Plac + PchBA + AraCpBAD + BSMT1

This was the first device employed in the project B.Fruity to inhibit the ripening maturation with the production of Methyl Salicylate. It contains all the enzymes needed to produce MeSA by the metabolic intermediate chorismate present both in E.coli and B.Subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3107
    Illegal BamHI site found at 3856
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1678
    Illegal AgeI site found at 2942
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3397
    Illegal SapI site found at 2924


[edit]
Categories
Parameters
None