Translational_Unit

Part:BBa_K1065104:Design

Designed by: Gabriele Girelli   Group: iGEM13_UNITN-Trento   (2013-08-13)
Revision as of 13:47, 14 August 2013 by Ggirelli (Talk | contribs) (→‎Design Notes)


RBS + SAM synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 696
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene sequence was extracted using the following primers:


>Primer Forward: prefix + RBS + spacer + ATG...

GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT

>Primer Reverse: sequence_TAATAA_suffix

GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT

Source

SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.

References