Translational_Unit

Part:BBa_K1065104:Design

Designed by: Gabriele Girelli   Group: iGEM13_UNITN-Trento   (2013-08-13)


RBS + SAM synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 116
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 696
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene sequence was extracted using the following primers:


>Primer Forward: prefix + RBS + spacer + ATG...

GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT

>Primer Reverse: sequence_TAATAA_suffix

GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT

Source

SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.

References

  1. Ethylene biosynthesis and its regulation in higher plants
    Yang, S. F.; Hoffman N. E. Ann. Rev. Plant Physiol. 1965, 35, 155-89.
  2. MetaCyc EC 2.5.1.6, MetaCyc. August 15th, 2013, from link
  3. Ethylene biosynthesis and signaling networks
    Wang, K. L.-C.; Li, H.; Ecker J. R. Plant Cell 2002, Supplement, S131-S151