Coding
RhaS

Part:BBa_K902068

Designed by: Chris Wintersinger   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 20:30, 28 October 2012 by Cmwinter (Talk | contribs)

RhaS

RhaS is a transcription factor which is part of the rhaSR operon.

Usage and Biology

This serves as regulatory element which activates expression of the pBAD operon on the pRha rhamnose promoter. In order for it to bind to pRha, the crp-cAMP complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).

In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the RhaR-rhamnose complex (Egan & Schlief, 1993).

Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None